cytoprotective effects of hydrophilic and lipophilic extracts of pistacia vera against oxidative versus carbonyl stress in rat hepatocytes

نویسندگان

jafar shahraki department of pharmacology and toxicology, faculty of pharmacy, zabol university of medical sciences, zabol, iran

mona zareh faculty of pharmacy, shahid beheshti university of medical sciences, tehran, p.o. box 14155-6153, iran

mohammad kamalinejad faculty of pharmacy, shahid beheshti university of medical sciences, tehran, p.o. box 14155-6153, iran

jalal pourahmad -

چکیده

this study was conducted to evaluate the cytoprotection of various extracts and bioactive compounds found in pistacia vera againts cytotoxicity, ros formation, lipid peroxidation, protein carbonylation , mitochondrial and lysosomal membrane damages in cell toxicity models of diabetes related carbonyl (glyoxal) and oxidative stress (hydroperoxide). methanol, water and ethyl acetate were used to prepare crude pistachios extracts, which were then used to screen for in vitro cytoprotection of freshly isolated rat hepatocytes against these toxins. the order of protection by pistacia vera extracts against both hydroperoxide induced oxidative stress (ros formation) and glyoxal induced protein carbonylation was: pistachio methanolic extract >pistachio water extract, gallic acid, catechin> α-tochoferol and pistachio ethyl acetate extract . finally due to higher protection achieved by methanolic extract even compared to sole pretreatment of gallic acid, catechin or α-tochoferol, we suggest that cytoprotection depends on the variety of polar and non-polar compounds found in methanolic extract, it is likely that multiple cytoprotective mechanisms are acting against oxidative and carbonyl induced cytotoxicity. to our knowledge, we are the first to report the cytoprotective activity of pistacia vera extracts against oxidative and carbonyl stress seen in type 2 diabetes hepatocytes model.

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منابع مشابه

Cytoprotective Effects of Hydrophilic and Lipophilic Extracts of Pistacia vera against Oxidative Versus Carbonyl Stress in Rat Hepatocytes

This study was conducted to evaluate the cytoprotection of various extracts and bioactive compounds found in Pistacia vera againts cytotoxicity, ROS formation, lipid peroxidation, protein carbonylation , mitochondrial and lysosomal membrane damages in cell toxicity models of diabetes related carbonyl (glyoxal) and oxidative stress (hydroperoxide). Methanol, water and ethyl acetate were used to ...

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Cytoprotective Effects of Hydrophilic and Lipophilic Extracts of Pistacia vera against Oxidative Versus Carbonyl Stress in Rat Hepatocytes

This study was conducted to evaluate the cytoprotection of various extracts and bioactive compounds found in Pistacia vera againts cytotoxicity, ROS formation, lipid peroxidation, protein carbonylation , mitochondrial and lysosomal membrane damages in cell toxicity models of diabetes related carbonyl (glyoxal) and oxidative stress (hydroperoxide). Methanol, water and ethyl acetate were used to ...

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Cytoprotective Effects of Hydrophilic and Lipophilic Extracts of Pistacia vera against Oxidative Versus Carbonyl Stress in Rat Hepatocytes

This study was conducted to evaluate the cytoprotection of various extracts and bioactive compounds found in Pistacia vera againts cytotoxicity, ROS formation, lipid peroxidation, protein carbonylation, mitochondrial and lysosomal membrane damages in cell toxicity models of diabetes related carbonyl (glyoxal) and oxidative stress (hydroperoxide). Methanol, water and ethyl acetate were used to p...

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عنوان ژورنال:
iranian journal of pharmaceutical research

جلد ۱۳، شماره ۴، صفحات ۱۲۶۳-۱۲۷۷

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